Method of producing hepatitis B surface antigen

Details Method of producing hepatitis B surface antigen Method of producing hepatitis B surface antigen

1979-11-02

1981-11-17

Naff, David M.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 68, 435285, C12N 502, C12P 2100, C12M 304

Patent

active

043012507

ABSTRACT:
Hepatitis B surface antigen (HB.sub.s Ag) is produced in vitro in high titer and purity from tissue cultures of cells that shed HB.sub.s Ag by growing the cells with a first incubation at elevated temperature followed by a second incubation at a lower elevated temperature.

REFERENCES:
patent: 3821087 (1974-06-01), Knazek et al.
Alexander, et. al., Studies on In Vitro Production of Hepatitis B Surface Antigen by a Human Hepatoma Cell Line, Perspectives in Virology, vol. 10, 1978, (pp. 103-120).

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