Method of producing glucosylated saccharides with kojibiose phos

Details Method of producing glucosylated saccharides with kojibiose phos Method of producing glucosylated saccharides with kojibiose phos

1998-11-10

2000-05-23

Weber, Jon P.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 72, C12P 1944, C12P 1900

Patent

active

060664770

ABSTRACT:
A kojibiose phosphorylase which hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and .beta.-D-glucose-1-phosphoric acid, forms kojibiose and an inorganic phosphoric acid from .beta.-D-glucose-1-phosphoric acid, and catalyzes the transfer reaction of glucosyl group to other saccharides using .beta.-D-glucose-1-phosphoric acid as a saccharide donor. The enzyme is obtainable from natural sources such as microorganisms of the genus Thermoanaerobium, and obtainable by recombinant technology.

REFERENCES:
patent: Re33047 (1989-09-01), Miyake et al.
patent: 4521252 (1985-06-01), Miyake et al.
Hatt et al (eds.), Catalogue of Bacteria and Phages, 18th Ed., American Type Culture Collection, USA (1992).
Sambrook et al, Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press (1989).
Enzyme Handbook, Asakura-Shoten Publisher, Tokyo, Japan (1982).

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