Method of producing .gamma.-globulin for intravenous injection a

Drug – bio-affecting and body treating compositions – Fermentate of unknown chemical structure – Having a known elemental analysis

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424177, 435 68, 435188, 435272, 260112B, C07G 700

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active

044367244

ABSTRACT:
A method of producing .gamma.-globulin which can be administered intravenously without adverse reactions and loss of opsonic activity is provided. The method comprises treating .gamma.-globulin with pepsin or uropepsin in a neutral pH range of 6.0 to 7.5. The aggregates in .gamma.-globulin are selectively decomposed by the method of the present invention, while any decomposition of monomer .gamma.-globulin molecule is substantially prevented. A therapeutic agent for intravenous injection which is reduced its anticomplementary activity and is stabilized by adding uropepsin which serves simultaneously as a proteolytic enzyme and a stabilizer, to human .gamma.-globulin. Uropepsinogen can be also as a stabilizer.

REFERENCES:
patent: 3553317 (1971-01-01), Michaelson et al.
patent: 4093606 (1978-06-01), Coval
patent: 4312949 (1982-01-01), Ahrens
Methods in Enzymology, vol. XIX pp. 406-421 (1970), Perlmann et al.
Vox Sang. vol. 13, No. 1 (1967), pp. 93-103, Koblet et al. and Steinbuch et al.

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