Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-28
1997-07-08
Ziska, Suzanne E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 435244, 536 231, 536 241, C12N 1500, C12N 500, C12N 138, C12P 2106
Patent
active
056460139
ABSTRACT:
A method of producing foreign gene products which comprises (1) transforming animal cells either (a) by using two plasmids, namely a plasmid containing the MMTV LTR and a foreign gene encoding a physiologically active substance inserted therein downstream from the LTR and a plasmid containing the LTR and a glucocorticoid receptor protein gene inserted therein downstream from the LTR, or (b) by using one plasmid comprising the LTR, a glucocorticoid receptor protein gene located downstream from the LTR, another LTR and a foreign gene encoding a physiologically active substance located downstream from the latter LTR, and (2) cultivating the transformed animal cells in a medium containing glucocorticoid in an amount effective for inducing mRNA transcription.
REFERENCES:
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Ko et al., Gene 84 (1989).
Lee et al., Nature 294:228-232 (1981).
Hynes et al., PNAS 80:3637 (1983).
Majors & Varmus, PNAS 80:5866-5870 (1983).
Vanderbilt et al., Chem. Abs. vol. 107:89:70998h (1987).
Meisfeld et al., Cell 46:389-399 (1986).
Ko Minoru S.H.
Takano Toshiya
Daiichi Seiyaku Co., Ltd.
Ziska Suzanne E.
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