Method of producing foreign gene products

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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4351723, 935 6, 935 32, 935 34, 935 36, 536 231, 536 241, C12N 1500, C12P 2106, C07H 2104

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055344194

ABSTRACT:
A method of producing foreign gene products which comprises (1) transforming animal cells either (a) by using two plasmids, namely a plasmid containing the MMTV LTR and a foreign gene encoding a physiologically active substance inserted therein downstream from the LTR and a plasmid containing the LTR and a glucocorticoid receptor protein gene inserted therein downstream from the LTR, or (b) by using one plasmid comprising the LTR, a glucocorticoid receptor protein gene located downstream from the LTR, another LTR and a foreign gene encoding a physiologically active substance located downstream from the latter LTR, and (2) cultivating the transformed animal cells in a medium containing glucocorticoid in an amount effective for inducing mRNA transcription.

REFERENCES:
patent: 4374927 (1983-02-01), Sninsky
Lee et al Nature 294: 228, 1981.
Miesfeld et al. Cell 46: 389, 1986.
Hynes et al PNAS 80: 3637, 1983.
Mayers et al PNAS 80: 5866, 1983.
Vanderbret et al Abstract CA 107: 70998h, from Mol. Endo. 1(1): 68-74, 1987.
Ko & Takano, DNA 8, 127 (1989).
Ko et al., Gene 84, 383 (1989).

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