Method of producing fermented sword beans

Details Method of producing fermented sword beans Method of producing fermented sword beans

1999-06-04

2000-10-10

Lilling, Herbert J.

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Conjugate or complex

435 41, A01N 6500

Patent

active

061299197

ABSTRACT:
A method of producing fermented sword beans to obtain composition of the sword bean that has inhibition action on cancer cells. The method includes steps of selecting proper sword beans and soaking the sword beans in an adequate amount of water, disinfecting the sword beans under high pressure and high temperature of 105.degree. C. for one hour, letting the sword beans cool to 38.degree. C., well mixing the sword beans with Aspergillus oryzae of 1.5/1000% in concentration, allowing the mixture to ferment in three separated stages for total 80 hours, adding water of five times in volume of the fermented mixture and well mixing them, extracting to obtain clear solution of the fermented sword beans, and freeze-drying the solution into powder. Tests conducted in laboratory indicate that liver cancer cells, HepG2, have a viability dropped by 80% when treated with the fermented sword bean solution of 200 .mu.g/ml in concentration, and zero when treated with the same solution of 400 .mu.g/ml in concentration, while the viability of normal liver cell line, Chang, is only affected by about 10%.

REFERENCES:
patent: 6004558 (1999-12-01), Thurn et al.
Computer Abstract BIOSIS 1984:349509 Jokl et al "Nutritive Values of Leaf Protein Concentrates From Tropical Legumes and From Leaves of Forest Trees" NTUR Rep INT (1984) 30 (1) 87-94.
Computer Abstract Caplus 1987:99542 Ozaki "Plant Regeneration From Hyupocotyl and Shoot Meristem Culture of Sword Bean (Canavalia Gladiata)" Shokubusu Soshiki Baiyo (1986) 3(2) 78-82.

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