Method of producing ectoprotein of hepatitis C virus

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4352402, 4353201, 530350, 536 221, 536 231, 536 2372, C12P 2106, C12N 500, C07K 100, C07H 1900

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058306919

ABSTRACT:
A method for extracellularly producing an ectoprotein of hepatitis C virus comprises the steps of cultivating a transformant which is transformed with an expression vector containing a DNA fragment coding for the ectoprotein of hepatitis C virus and recovering the ectoprotein of hepatitis C virus extracellularly produced by the transformant. The protein originated from the E1 region prepared by the method can be used as a material for preparing a vaccine for preventing HCV infection. In addition, a diagnostic agent containing the protein is useful for the detection of an HCV antibody or the confirmation of the presence thereof in sera or the like. In other words, the protein of the present invention permits the diagnosis of C type hepatitis in high specificity and sensitivity.

REFERENCES:
patent: 5302507 (1994-04-01), Chiba et al.
Biochemical and Biophysical Research Communications, vol. 175, No. 1, Feb. 28, 1991, pp. 220-228, Makoto Hijikata, et al., "Hypervariable Regions in the Putative Glycoprotein of Hepatitis C Virus".
Science, vol. 238, Dec. 18, 1987, pp. 1704-1707, D.H. Smith, et al., "Blocking of HIV-1 Infectivity by a Soluble, Secreted Form of the CD4 Antigen".
Journal of Virology, vol. 66, No. 3, Mar. 1992, pp. 1425-1431, Yoshiharu Matsuura, et al., "Expression of Processed Envelope Protein of Hepatitis C Virus in Mammalian and Insect Cells".
Proc. Natl. Acad. Sci., vol. 88, Jul. 1991, pp. 5547-5551, M. Hijikata, et al., "Gene Mapping of the Putative Structural Region of the Hepatitis C Virus Genome by in vitro Processing Analysis".
Genes. "Expression of cloned genes in animal cells" Gen cloning pp. 199-218 .

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