Method of producing double low restorer lines of Brassica...

Multicellular living organisms and unmodified parts thereof and – Method of using a plant or plant part in a breeding process... – Method of breeding involving a genotypic or phenotypic marker

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C800S274000, C800S306000

Reexamination Certificate

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07812217

ABSTRACT:
The invention relates to a method of producing a double low restorer line ofBrassica napusfor Ogura cytoplasmic male sterility (cms) presenting a radish introgression carrying the Rfo restorer gene deleted of the radish Pgi-2 allele and recombined with the Pgi-2 gene fromBrassica oleracea, and having a good agronomic value characterized by female fertility, a good transmission rate of Rfo and a high vegetative vigour. The invention relates also to a method of formingBrassica napushybrid seeds and progeny thereof and to the use of markers for selection.

REFERENCES:
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Primard-Brisset et al. Theoretical and Applied Genetics 11: 736-746 (2005).
Bartkowiak-Broda et al. 1999. Proceedings of the 10thInternational Rapeseed Congress, Camberra, Australia, pp. 1-5.
Delourme et al. 1999. Proceedings of the 10thInternational Rapeseed Congress, Camberra, Australia, pp. 6-9.
Desloire et al., “Identification of the fertility restoration locus,Rfo, in radish, as a member of the pentatricopeptide-repeat protein family”, EMBO reports vol. 4, No. 6, pp. 588-594, Jun. 6, 2003.
Delourme et al., Characterisation of the radish introgression carrying theRfo, restorer gene for theOgu-INRA cytoplasmic male sterility in rapeseed (Brassica napusI.), Theoretical and Applied Genetics, vol. 97, No. 1-2, pp. 129-134, Jul. 1998.
Delourme et al., Linkage between an isozyme marker and a restorer gene in radish cytoplasmic male sterility of rapeseed (Brassica napusL.), Theoretical and Applied Genetics, vol. 85, pp. 222-228, Springer, Berlin, DE, 1992.
Delourme et al., Identification of RAPD markers linked to a fertility restorer gene for the Ogura radish cytoplasmic male sterility of rapeseed (Brassica napusL.), Theoretical and Applied Genetics, vol. 88, No. 6/7, pp. 741-748, 1994, Springer, Berlin, DE.
Bellaoui et al., “The restorerRfogene acts post-translationally on the stability of the ORF138 CMS-associated protein in reproductive tissue of rapeseed cybrids”, vol. 40, No. 5, pp. 893-902, Jul. 1999, Plant Molecular Biol, NIJHOFF publishers, Dordrecht, NL.
Giancola et al., “Characterization of a radish introgression carrying the Ogura fertility restorer geneRfoin rapeseed, using theArabidopsisgenome sequence and radish genetic mapping”, TAG. Theoretical and Applied Genetics.vol. 107, No. 8, pp. 1442-1451, Aug. 27, 2003 Germany.
Fourmann et al., “FromArabidopsis thalianatoBrassica napus: development of amplified consensus genetic markers (ACGM) for construction of a gene map”, Theor . Appl. Genet, vol. 105, pp. 1196-1206, 2002.
Delourme et al., “Double Low Restored F1 Hybrids Can Be Produced With the Ogu-INRA CMS in Rapeseed”,10thRapeseed Congress, Canaberra 1999, (pp. 26-29).

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