Method of producing cloned animals by demecolcine treatment

Details Method of producing cloned animals by demecolcine treatment Method of producing cloned animals by demecolcine treatment

2008-01-03

2009-10-13

Bertoglio, Valarie (Department: 1632)

Multicellular living organisms and unmodified parts thereof and

Method of making a transgenic nonhuman animal

Via microinjection of a nucleus into an embryo, egg cell, or...

C800S014000, C435S375000, C435S377000

Reexamination Certificate

active

07601884

ABSTRACT:
A cloned animal is produced by demecolcine treatment characterized by culturing the nuclear transfer embryo in vitro and transferring the embryo in vivo. This significantly improves in vitro development of somatic cell nuclear transfer embryos and maintenance of pregnancy from transfer of transfer somatic cell nuclear transfer embryos in a surrogate mother up to delivery. A nuclear transfer embryo of a non-primate mammal made by enucleating a recipient oocyte; injecting a nuclear donor cell into the enucleated oocyte; fusing together the injected enucleated oocyte with the injected nuclear donor cell to form a fused oocyte; activating the fused oocyte; and treating the activated oocyte with demecolcine to form the nuclear transfer embryo.

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