Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Patent
1995-05-22
2000-04-11
Myers, Carla J.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
435455, 435461, 435352, 435366, 435360, 435357, 435 691, 435 694, 435 701, 536 235, C12N 1585, C07H 2104
Patent
active
060487240
ABSTRACT:
The present invention relates to transfected primary and secondary somatic cells of vertebrate origin, particularly mammalian origin, transfected with exogenous genetic material (DNA) that encodes erythropoietin or an insulinotropin (e.g., derivatives of glucagon-like peptide 1 (GLP-1)), methods by which primary and secondary cells are transfected to include exogenous genetic material encoding erythropoietin or an insulinotropin, methods of producing clonal cell strains or heterogenous cell strains that express erythropoietin or an insulinotropin, methods of gene therapy in which the transfected primary or secondary cells are used, and methods of producing antibodies using the transfected primary or secondary cells. The present invention includes primary and secondary somatic cells, such as fibroblasts, keratinocytes, epithelial cells, endothelial cells, glial cells, neural cells, formed elements of the blood, muscle cells, other somatic cells that can be cultured and somatic cell precursors, which have been transfected with exogenous DNA encoding erythropoietin or an insulinotropin, which is stably integrated into their genomes or is expressed in the cells episomally.
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Heartlein Michael W.
Selden Richard F
Treco Douglas
Myers Carla J.
Transkaryotic Therapies Inc.
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