Method of producing cell extract for cell-free protein...

Details Method of producing cell extract for cell-free protein... Method of producing cell extract for cell-free protein...

2005-03-02

2010-11-23

Noakes, Suzanne M (Department: 1656)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

C435S041000, C435S068100, C435S069100, C435S070100, C435S071100, C530S413000

Reexamination Certificate

active

07838640

ABSTRACT:
A cell extract for cell-free protein synthesis is produced by removing substances, which bind to an affinity support to be used in purification or interaction analysis, from a cell extract having protein synthetic activity. Then, a target protein is synthesized by using the cell extract for cell-free protein synthesis. The synthesized target protein can be purified by using the affinity support and used in interaction analysis.

REFERENCES:
patent: 5-236986 (1993-09-01), None
patent: 7-203984 (1995-08-01), None
Hay et al. (Cell, vol. 89, 149-158, Apr. 4, 1997).
Gonzalez et al. (A Novel Interaction between Perlecan Protein Core and Progranulin, The Journal of Biological Chemistry, vol. 278, No. 40, Issue of Oct. 3, pp. 38113-38116, 2003).
Madin et al. (A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: Plants apparently contain a suicide system directed at ribosomes, PNAS, Jan. 18, 2000, vol. 97, No. 2, pp. 559-564).
Zacharias et al. (Recombinant-protein solubility screening using the EasyXpress in vitro translation system, QIAGEN News 2004 e6, Retrieved from the Internet <URL:www1.qiagen.com/literature/qiagennews/weeklyArticle/04—02/e6/default.aspx>).
Greene et al. (The Journal of Biological Chemistry vol. 278, No. 9, Issue of Feb. 28, pp. 7617-7623, 2003).
Kimple et al. (Overview of affinity tags for protein purification, Curr Protoc Protein Sci., Sep. 2004, Chapter 9: Unit 9.9).

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