Method of producing cell extract for cell-free protein...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S041000, C435S068100, C435S069100, C435S070100, C435S071100, C530S413000

Reexamination Certificate

active

07838640

ABSTRACT:
A cell extract for cell-free protein synthesis is produced by removing substances, which bind to an affinity support to be used in purification or interaction analysis, from a cell extract having protein synthetic activity. Then, a target protein is synthesized by using the cell extract for cell-free protein synthesis. The synthesized target protein can be purified by using the affinity support and used in interaction analysis.

REFERENCES:
patent: 5-236986 (1993-09-01), None
patent: 7-203984 (1995-08-01), None
Hay et al. (Cell, vol. 89, 149-158, Apr. 4, 1997).
Gonzalez et al. (A Novel Interaction between Perlecan Protein Core and Progranulin, The Journal of Biological Chemistry, vol. 278, No. 40, Issue of Oct. 3, pp. 38113-38116, 2003).
Madin et al. (A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: Plants apparently contain a suicide system directed at ribosomes, PNAS, Jan. 18, 2000, vol. 97, No. 2, pp. 559-564).
Zacharias et al. (Recombinant-protein solubility screening using the EasyXpress in vitro translation system, QIAGEN News 2004 e6, Retrieved from the Internet <URL:www1.qiagen.com/literature/qiagennews/weeklyArticle/04—02/e6/default.aspx>).
Greene et al. (The Journal of Biological Chemistry vol. 278, No. 9, Issue of Feb. 28, pp. 7617-7623, 2003).
Kimple et al. (Overview of affinity tags for protein purification, Curr Protoc Protein Sci., Sep. 2004, Chapter 9: Unit 9.9).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method of producing cell extract for cell-free protein... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of producing cell extract for cell-free protein..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of producing cell extract for cell-free protein... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4229035

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.