Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-06-05
1997-02-04
Rollins, John W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435103, 435829, 53612312, 536124, C12P 1904, C12P 1908
Patent
active
055996973
ABSTRACT:
The present invention comprises a method of producing .beta.-1,3-glucan wherein a microbe capable of producing .beta.-1,3-glucan is cultured in a medium containing as a nitrogen source an ammonium salt of an organic carboxylic acid the microbe can assimilate wherein the .beta.-1,3-glucan is produced and accumulated, and then harvesting. The .beta.-1,3-glucan production method of the present invention does not require addition of a pH regulator such as calcium carbonate or alkali ions, since the medium does not decrease in pH because a microbe capable of producing .beta.-1,3-glucan is cultured in a medium containing as a nitrogen source an ammonium salt of an organic carboxylic acid which the microbe can assimilate. For this reason, cultivation is not adversely affected, nor does any salt form in the medium, aspects which facilitate purification of said glucan, offering an industrially excellent production method. Moreover, the method of the present invention makes it feasible to produce .beta.-1,3-glucan at high productivity and high yield relative to sugar.
REFERENCES:
patent: 3754925 (1973-08-01), Kimura et al.
patent: 4355106 (1982-10-01), Lawford
"Agricultural Biological Chemistry, Growth and .beta.-Glucan 10C3K Production by a Mutant of Alcaligenes faecalis var. myxogenes in Defined Medium" Harada et al., vol. 30, No. 8, pp. 764-769, 1966.
Kanegae Yukihiro
Kimura Kazutsugu
Nakatsui Isamu
Prats Francisco C.
Rollins John W.
Takeda Chemical Industries Ltd.
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