Method of producing antibodies to HPV

Details Method of producing antibodies to HPV Method of producing antibodies to HPV

1989-03-15

1991-09-03

Nucker, Christine

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 792, 436548, 935110, 422 61, C12Q 102, G01N 33569

Patent

active

050454471

ABSTRACT:
A method for the production of antibodies to an immunogen, wherein hybridoma culture supernatants are screened using cells infected with a virus recombinant encoding one or more epitopes of the immunogen.
The method disclosed is particularly advantageous as: 1) cells infected with the virus recombinant can provide a good supply of an antigen; 2) the antigen is expressed in a mammalian cell and it is therefore synthesized and processed in a form essentially identical to the antigen found in a natural infection; and 3) the infected cells may be subjected to the same pre-treatments as applied to test sample tissues before their use in the hybridoma screening procedure. This ensures that the antibodies selected will recognize epitopes resistant to these pre-treatments and which may therefore be expected to be present on the test samples as prepared for analysis.

REFERENCES:
patent: 4777239 (1988-10-01), Schoolnik et al.
Banks, et al., J. Gen. Virol., 68, pp. 3081-3089, 1987.
U.S. Ser. No. 58,387, 7/43, H. V. Gelbain, et al., abstract only.
Browne, et al., J. Gen. Virol., 69, pp. 1263-1273, 1988.
Doorbar, et al., J. Virol., 61(9), 2793-2799, 1987.
Kieng, et al., Biotechnology, vol. 4, 790-795, 1986.
Chakrabarti, et al., Nature, vol. 320, 535-537, 1986.
J. C. Sterling, et al., J. Cell Biochem. Supplement, No. 13, Part C, Dec. 1989 (U.S.).
J. C. Sterling, et al., New Mexico Symposium on Papillomaviruses, Mar. 11-18, 1989.
Shepherd BSI Abstract, Nov. 1988.
D. Espion, et al., Arch. Virol., 1987, 95 (1-2), pp. 79-95, abstract only.
Doorbar, et al., Embo. J., 8(3), p. 825 (1988).
Schneider-Gadicke, Cancer Res. (1988) 48, 2969-2974.
Ottersdorf, J. Gen. Virol. (1987) 68, 2933.

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