Method of producing an R-type bacteriocin and its use in the det

Details Method of producing an R-type bacteriocin and its use in the det Method of producing an R-type bacteriocin and its use in the det

1976-03-31

1979-03-06

Wiseman, Thomas G.

Textiles: fiber preparation

1951035A, 1951035M, 195104, 195122, C12D 1306, G01N 3100, C12K 104

Patent

active

041429397

ABSTRACT:
A method of detecting a specific microorganism comprising contacting said microorganism with bacteriocins from a microorganism of a genus which is taxonomically unrelated to said specific organism. The result of such contact may be utilized to detect the presence of a microorganism belonging to a taxonomically unrelated genus. Radio-labeled or fluorescein-labeled bacteriocins can be reacted with specific bacteria in a biological sample and the presence of such specific bacteria detected by removing excess bacteriocins and determining the presence of fluorescent or radioactive bacteria in the sample. Neisseria gonorrhoeae is identified by spotting bacteriocins on a plate of clinical material; or using a disk impregnated with bacteriocins placed on a plate inoculated with the clinical material; or the bacteriocins can be incorporated into one-half of a split agar plate, the identification being made on the basis of a zone of inhibition surrounding the spot where the bacteriocins were applied, or growth inhibition on the portion of the plate to which the bacteriocins were added.

REFERENCES:
kanchukh, et al., "Determination of Pesticin and Antibodies to it in the roprecipitation Reaction", Chemical Abstracts, vol. 83, No. 9, p. 361 (1975) Abs. No. 75150w.
Jetten, et al. "Characteristics of the Killing Effect of a Staphylococcus epidermidis Bacteriocin", Chemical Abstracts, vol. 81, No. 5, p. 100 (1974) Abs. No. 21627x.
Jetten, et al., "Characterization and Extrachromosomal Control of Bacteriocin Production in Staphylococcus aureus", Chemical Abstracts, vol. 79, No. 23, p. 139 (1973) Abs. No. 134162u.
Walstad, et al., "Growth Inhibition among Strains of Neisseria gonorrhoeae Due to Production of Inhibitory Free Fatty Acids and Lysophosphatidylethanolamine, Absence of Basteriocins," Chemical Abstracts, vol. 81, No. 25, p. 256 (1974), Abs. No. 165950v.
Ito, et al., "Isolation and Characterization of Pyocins from Several Strains of Pseudomenas aeruginosa", J. Gen. Appl. Microbiol., vol. 16, (1970), pp. 205-214.
Jetten, et al., "Production and Purification of a Staphylococcus epidermidis Bacteriocin," J. of Bact., vol. 112, No. 1, (1972) pp. 235-242.
Jetten et al., "Nature and Properties of a Staphylococcus epidermidis Bacteriocin", J. of Bact., vol. 112, No. 1, (1977), pp. 243-250.
Reeves, "The Bacteriocins", Bacteriol. Rev., vol. 29, No. 1, Am. Soc. Microbiology, (1965), pp. 24-45.

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