Method of producing an erythroid cell which is...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S006120, C435S007100, C435S007250, C435S320100, C435S325000

Reexamination Certificate

active

07033784

ABSTRACT:
A method of producing an erythroid cell which is substantially undifferentiated but which is capable of expressing a heterologous protein under the control of a globin promoter thereof, which method comprises maintaining growing uninduced erythroid cells in culture for sufficient period of time that the protein is expressed, and isolating a subclone which expresses said protein. Erythroid cells produced by the method, and methods of detecting the interaction of an insect G-protein coupled receptor with an endogenous signaling cascade of erythroid cells are also described and claimed.

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patent: WO 92/11380 (1992-07-01), None
patent: WO 98/35020 (1998-08-01), None
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Garcia-Alonso M. et al. “Stable Functional Expression of the Neuronal Nicotinic Acetylcholine Receptor α3β4 in MEL (Murine Erythroleukemia) Cells: A Novel Expression System for Ligand Gated Ion Channels”,Society for Neuroscience Abstracts, vol. 22, p. 1526 (1996).
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