Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1991-09-30
1994-02-22
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 43525234, C12P 2100, C12N 1500, C12N 121
Patent
active
052886174
ABSTRACT:
Antigenic preparations active against species of bacteria naturally producing Type 4 fimbriae, such as Bacteroides nodosus which causes footrot in sheep, are produced by culturing a suitable genetically engineered aerobic or facultatively anaerobic host bacterial cell such as Pseudomonas aeruginosa. The host cell contains the gene encoding the fimbrial structural subunit antigens of at least one strain of the said species of bacteria and an endogenous compatible system for the morphogenetic assembly of Type 4 fimbriae and/or the genes for the morphogenetic assembly of such fimbriae derived from a Type 4 fimbriate species. The genetically engineered host cells are cultured such that nature fimbriae are produced as extracellular structures and the fimbriae are harvested, substantially free of the host cells, for use as the antigenic preparation. In preferred embodiments of the invention the natural fimbrial subunit gene is modified to enhance expression in the new host. Antigenic preparations according to this invention have proven to be effective vaccines against footrot.
REFERENCES:
patent: 4696896 (1987-09-01), Brinton
patent: 4769240 (1988-09-01), Brinton
Biological Abstracts; vol. 7(3); Feb. 1, 1984; 19281; Lee et al. 1983 Am. J. Vet. Res. 44(9):1676-81.
Elleman et al. 1984, FEBS Lett. 173(1):103-107.
Paranchych et al, Can. J. Microbiol. 25:1175-1181 (1979).
Froeholm et al. FEBS Lett. 73(1):29-32 (1977).
Elleman, T. C. et al. "Expression of Multiple Types . . . " Molecular Microbiology, 1987 1(3), 377-380.
Elleman, T. C. et al. "Isolation of the Gene . . . " Chemical Abstracts 101:145092v, vol. 101, 1984, p. 166.
Anderson, B. J. et al. "Cloning and Expression . . . " 3-Biochem Genetics 102:18866w, vol. 102, 1985, pp. 189-190.
Anderson Belinda J.
Elleman Thomas C.
Mattick John S.
Commonwealth Scientific and Industrial Research Organization
Schwartz Richard A.
University of Sydney
Vogel Nancy
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