Method of producing a thermostable DNA polymerase from Thermus t

Details Method of producing a thermostable DNA polymerase from Thermus t Method of producing a thermostable DNA polymerase from Thermus t

1992-06-05

1993-09-07

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C12N 1112

Patent

active

052428184

ABSTRACT:
A DNA polymerase with excellent thermostability is provided. The DNA polymerase retains at least about 60% of its activity when placed at pH 8.0 for 2 hours at 85.degree. C.

REFERENCES:
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R. K. Saiki et al., "A Novel Method for the Detection of Polymorphic Restriction Sites by Cleavage of Oilgonucleotide Probes: Application to Sickle-Cell Anemia," Bio/Technology, 3, 1008-1012 (1985).
C. Ruttiman et al., "DNA polymerases from the extremely thermophilic bacterium Thermus thermophilus HB-8," European Journal of Biochemistry, 149, 41-46 (1985).
A. Chien et al., "Deoxyribonucleic Acid Polymerase from the Extreme Thermophile Thermus aquaticus," Journal of Bateriology, vol. 127 No. 3, pp. 1550-1557 (Sep. 1976).
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Elie et al. (1989) Eur. J. Biochem., 178(3), 619-626.
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