Method of producing a physical map contigous DNA sequences

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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4351723, C12Q 168, C12N 1509

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055807348

ABSTRACT:
Materials and methods for homologous-recombination screening of DNA libraries constructed in a eukaryotic host and methods for homologous-recombination chromosome walking for isolating overlapping DNA sequences for building an extended physical map of a chromosomal region.

REFERENCES:
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Ma et al. "Plasmid construction by homologous recombination in yeast", Gene 58: 201-216. 1987.
Traver et al. "Rapid screening of a human genomic library in yeast artificial chromosomes for single -copy sequences", Proc. Natl. Acad. Sci. USA 86: 5898-5902. Aug. 1989.
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Struhl et al. "High-frequency transformation of yeast: autonomos replication of hybrid DNA molecules", Proc. Natl. Acad. Sci. USA 76: 1035-1039. 1979.
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Vijayakumar et al. (1986) Structure of a conjugative element in Streptococcus pneumoniae. J. Bacteriol. 166: 978-984.
Stiles et al. (1981) DNA sequence of a mutation in the leader region of the yeast Iso-1-cytochrome c mRNA Cell 25:277-284.
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Robins et al. (1981) Transforming DNA integrates into the host chromosome Cell 23: 29-39.

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