Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1996-07-23
1999-03-09
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
204400, 204403, 204415, 204416, 204418, 422 8201, 422 8203, 4352871, 435817, 436 71, 436518, 436528, 436806, 427 211, 427 213, 427 58, 427337, 427338, G01N 33553, G01N 27327
Patent
active
058798781
ABSTRACT:
Electrode membrane combinations for use in biosensors to detect analytes in a sample and methods for making and storing same are disclosed. In one aspect, a method is provided for producing a first layer electrode membrane comprising:
(1) Forming a solution containing Linker Lipid A, the disulfide of mercaptoacetic acid (MAAD) or similar molecule, linker Gramicidin B, membrane spanning lipid C (MSL-C) and membrane spanning lipid D (MSL-D) or other suitable linker molecules and other ion channel combinations;
(2) Contacting an electrode containing a clean gold surface with the solution, the disulfide containing components in the solution thus adsorbing onto the gold surface of the electrode;
(3) Rinsing the electrode with a suitable organic solvent; and
(4) Removing the excess organic solvent used for rinsing.
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Braach-Makavytie Vijoleta Licija
Cornell Bruce
King Lionel George
Pace Ronald John
Raguse Burkhard
Australian Membrane and Biotechnology Research Institute
Chin Christopher L.
The University of Sydney
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