Method of producing a cell carrying an excess of mammalian centr

Details Method of producing a cell carrying an excess of mammalian centr Method of producing a cell carrying an excess of mammalian centr

1996-10-21

1999-04-06

Railey, II, Johnny F.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435325, 435352, 435354, C12N 510

Patent

active

058916911

ABSTRACT:
DNA fragments and methods for obtaining them are disclosed which when put into mammalian cells together with a dominant marker gene are able to form functional centromeres. The sequences can be used to generate probes for these centromeres. Cell lines containing the functional centromeres are also provided. Methods are taught for isolating mammalian centromeric DNA as well as for producing cell lines carrying an excess of mammalian centromeres linked to a dominant selectable marker gene.

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