Method of prevention of oxidative injury to cells

Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...

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424101, 4352402, 43524025, A01N 102, C12N 500

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active

048700029

ABSTRACT:
A process for preventing oxidative injury in which living cells are contacted with a protective amount of 3-amino-tyrosine or a derivative thereof. Also provided is a process for preserving a cell sample involving collecting a sample of cells and adding 3-aminotyrosine or a derivative thereof to that cell sample. A process for preventing thermal damage to living cells is also provided which comprises contacting the cells with a thermal protectant amount of 3-aminotyrosine or a derivative thereof, as is a process for protecting living cells from damage caused by ionizing radiation, which comprises contacting the cells with a radioprotectant amount of 3-aminotyrosine or a derivative thereof. Also provided is a process for suppressing anti-inflammatory reactions in animals which comprises administering an anti-inflammatory amount of 3-aminotrosine or a derivative thereof to an animal.

REFERENCES:
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Lefkowitz, Doris L. et al, "Activation of Macrophages with Oxidative Enzymes," Methods in Enzymology, vol. 132, 1986, pp. 537-548.
Kiel, Johnathan L. et al, "Physiologic Aging of Mature Porcine Erythrocyutes: Effects of Various Metabolites, Antimetabolites, and Physical Stressors," Am. J. Vet. Res., vol. 47, No. 10, Oct. 1986, pp. 2155-2160.
Kiel, Johnathan L. et al, "3-Amino-L-tyrosine and Its Derivatives as Anti-Inflammatory and Immunosuppressive Compounds," Medicine & Biology, 1987, p. 190.
Pruett, S. B. et al, "Relationship Between Oxidative Metabolism and Thiol Production in Macrophage-Like Cell Lines", Federation of American Societies for Experimental Biology, 72nd Annual Meeting, Las Vegas, Nevada, May 1-5, 1988, p. 3611.

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