Method of preventing virus increase in plants

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Conjugate or complex

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435 68, A61K 3578, C12P 2106

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active

045200203

ABSTRACT:
A method of isolating material which inhibits virus replication in plants comprising isolating protoplasts from a local lesion-responding tobacco plant having an N gene, preferably a Samsun NN tobacco plant, inoculating the protoplasts with Tobacco Mosaic Virus, and either removing the protoplasts after a predetermined amount of time and isolating the desired material from the protoplast incubation medium, or removing the virus after a predetermined amount of time and isolating the desired material from the preparation.
Also provided is a method of isolating material which inhibits virus replication in plants comprising isolating tissue from a "green island" area of a tobacco plant which responds systemically to Cucumber Mosaic Virus, preferably a Samsun NN, a Xanthi-nc or Samsun plant, homogenizing the tissue and isolating the desired material from the homogenate, as well as a method of immunizing plants against virus replication comprising applying thereto the material isolated as above.

REFERENCES:
I. Sela, Adv. in Virus Res., vol. 26: 201-237, 19.
I. Sela, et al., Virol., 89: 1-6, 1978.
G. Loebenstein et al., Virol., 100: 110-115, 1980.
Sela et al., Occurrence of Artiviral Factor in Virus Infected Plants, Virol., 17: 543-548, 1962.
Sela et al., Separation of a Highly Active Anti-Viral Factor from Virus-Infected Plants, Virol., 22: 446-451, 1964.

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