Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1985-05-03
1989-02-28
Moskowitz, Margaret
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435184, 4352401, 514885, C12N 500, C12N 999
Patent
active
048085332
ABSTRACT:
A cell mediated immune response system glycoprotein having a molecular weight of about 90,000 and having at least one sialic acid moiety as a biologically active site is disclosed. The glycoprotein is specifically bound by wheat germ agglutinin and also by the hydrophobically binding ligand Blue A (Cibacron Blue F3G-A) but does not bind to lysine. The glycoprotein is a necessary cofactor with Interleukin-1 in the biosynthesis of T-cell growth factor (I1-2). A process for producing a serum-free and mitogen-free I1-2 in vitro by adding the glycoprotein to a serum-free and mitogen-free interleukin-1 preparation is described. The method for producing the serum-free and mitogen-free Interleukin-1 is also described. A chemically defined T-cell growth culture medium containing the new glycoprotein as the only protein substance is used in the above process and also provides a means for studying regulation of T-cell lymphocyte growth. Inhibitors for the formation of T-cell blasts in cell mediated immunological response provide a powerful new tool for preventing host-graft rejection. These inhibitors are alkylating agents for serine esterase enzymes which have been found to be involved in the cell mediated immunological response system which generates natural Killer (NK) cells, the latter being responsible for graft rejection in a host.
REFERENCES:
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patent: 4681844 (1987-07-01), Fabricius
Chang et al., "Effects of N.alpha.-Tosyl-L-Lysyl-Chloromethyl-Keton on the Activity of Cytotoxic T Lymphocytes", Journal of Immunology, vol. 124(3), Mar. 1980, pp. 1028-1033.
Levy et al., "Effect of N-Acetylcystein on Some Aspects of Cyclophosphamide-Induced Toxicity and Immunosuppression", Biochemical Pharmacology, vol. 26, 1977, pp. 1015-1020.
Schwartz, A. et al., Journal of Immunology, 117(3); 871-875, (9-1976).
Fabricius Hans-Ake
Kottgen Eckart U.
Moskowitz Margaret
Shanksville Corporation NV
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