Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2006-05-02
2006-05-02
Riley, Jezia (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S025300, C536S026600
Reexamination Certificate
active
07038027
ABSTRACT:
The present invention relates to a method of preventing modification of a synthetic oligonucleotide or oligonucleotide analog during removal of at least one β-cyanoethyl protecting group from the oligonucleotide or oligonucleotide analog. The method involves contacting the oligonucleotide or oligonucleotide analog with a basic solution having at least one acrylonitrile scavenger, such as t-butylamine, at a sufficient temperature and for a sufficient period of time to remove at least one β-cyanoethyl protecting group. The present invention also relates to a method of producing a synthetic oligonucleotide or oligonucleotide analog.
REFERENCES:
patent: 4419509 (1983-12-01), Hsiung
patent: 5518651 (1996-05-01), Reddy et al.
patent: 5623068 (1997-04-01), Reddy et al.
patent: 5705621 (1998-01-01), Ravikumar
patent: 5750672 (1998-05-01), Kempe
patent: 5760209 (1998-06-01), Cheruvallath et al.
patent: 5783690 (1998-07-01), Cheruvallath et al.
patent: 5847106 (1998-12-01), Ravikumar et al.
patent: 5859232 (1999-01-01), Ravikumar
patent: 6465628 (2002-10-01), Ravikumar et al.
Sinha, N.D., et al., “Polymer support oligonucleotide synthesis XVIII1.2: use of β-cyanoethyl-N, N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product,”Nucleic Acids Research, 12(11) :4539-4557 (1984).
Eritja, R., et al., “A Synthetic Procedure for the Preparation of Oligonucleotides Without Using Ammonia and its Application for the Synthesis of the Oligonucleotides Containing O-4-AlkylThymidines,”Tetrahedron, 48(20) : 4171-4182 (1992).
Reese, C.B., et al., “Avoidance of sulfur loss during ammonia treatment of oligonucleotide phosphorothioates,” Nucleic Acids Research, 25(14): 2943-2944 (1997).
Hsiung, H., et al., “Further improvements on the phosphotriester synthesis of deoxyribooligonucleotides and the oligonucleotide directed site-specific mutagenesis ofE. colilipoprotein gene,”Nucleic Acids Research, 11(10) :3227-3239 (1983).
Avecia Biotechnology Inc.
Hamilton Brook Smith & Reynolds P.C.
Riley Jezia
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