Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1995-04-10
1997-10-21
Kim, John
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435260, C12N 500, C12N 506
Patent
active
056795653
ABSTRACT:
A method, a solution and a chamber for the preparation and storage of pancreatic islets. The method includes contacting a pancreas with a warm collagenase solution, digesting the pancreas in the warm collagenase solution to form warm digest, adding cold preservative solution to the warm digest, agitating the warm digest/cold preservative solution at a temperature between about 0.degree. and 15.degree. C., to thereby further digest the partially digested pancreas included in the warm digest, to form cold digest and collecting liquid from the cold digest to form isolated islets. The cold preservative solution and a pancreatic islet preservative solution of the present invention include D-mannitol, K-lactobionate and a buffer.
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Kenmochi Takashi
Mullen Yoko
Kim John
The Regents of the University of California
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