Method of preparing yeasts enriched in L-lysine and capable of e

Details Method of preparing yeasts enriched in L-lysine and capable of e Method of preparing yeasts enriched in L-lysine and capable of e

1975-04-21

1976-10-19

Monacell, A. Louis

Textiles: fiber preparation

195 79, C12B 100

Patent

active

039869334

ABSTRACT:
A method of preparing a yeast enriched in L-lysine and capable of excreting an organic acid, such as citric acid, and possibly also isocitric and glutamic acids, when cultivated on a hydrocarbon-containing medium comprises subjecting a yeast of the species Candida lipolytica to the action of a mutagenic agent, such as a chemical agent or radiation, cultivating the thus-treated yeast in a culture medium containing a lysine analogue, preferably that to which the original yeast is most sensitive, in a concentration which is about 50 to 100 times greater than that to which the cells of the original yeast are most sensitive, and cultivating the resultant mutated resistant yeast on a medium containing at least one hydrocarbon such as a C.sub.10 -C.sub.20 normal paraffin as well as conventional nutrients, the cultivation preferably being effected in an agitated aerated medium at a pH of 3 to 6 and a temperature of 25.degree. to 30.degree. C.. The yeast enriched in L-lysine may be recovered from the reaction medium as a useful product and citric acid and other acids may be recovered in conventional manner from the reaction medium.

REFERENCES:
patent: 3660235 (1972-05-01), Okumura et al.
patent: 3825472 (1974-07-01), Kubota et al.
patent: 3873424 (1975-03-01), Kimura et al.

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