Plant husbandry – Process – Manipulating seed or seedling
Reexamination Certificate
2007-09-18
2007-09-18
Palo, Francis T. (Department: 3644)
Plant husbandry
Process
Manipulating seed or seedling
Reexamination Certificate
active
10656496
ABSTRACT:
Disclosed is a method of preparingTricholoma matsutake-infected young pine trees by coculturing aseptic seedlings of a pine tree with aT. matsutakeisolate, comprising the steps of liquid-culturing fruit bodies of naturally occurringmatsutakemushrooms in PDB (Potatto Dextrose Broth, Difco) and inoculatingT. matsutakeKBFERI 20T05 isolated from the cultured fruit bodies; placing mixed soil of perlite andsphagnumpeatmoss and K-liquid medium onto the inoculated fungus; and planting aseptic seedlings obtained by aseptically germinating pine seeds into the mixed soil, and then culturing the pine seedlings with theT. matsutakeisolate to allow ectomycorrhiza formation on the fine roots of the pine seedlings. The method of the present invention is advantageous in terms of giving objectivity for the fungal inoculum by employing as an inoculum a fungus having DNA homology with the conventionally knownT. matsutake, and of making it possible to mass produceT. matsutake-infected pine trees throughout the Year.
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Cheon Woo-Jae
Park Moo-Chang
Sim Sang-Gab
Govenor of Gyeongsangbukdo
Mathews, Shepherd, McKay & Bruneau, P.A.
Palo Francis T.
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