Method of preparing specimen for counting subpopulations of leuk

Details Method of preparing specimen for counting subpopulations of leuk Method of preparing specimen for counting subpopulations of leuk

1992-06-23

1993-11-23

Housel, James C.

Chemistry: analytical and immunological testing

Biological cellular material tested

436 17, 436172, 436800, G01N 3348

Patent

active

052643697

ABSTRACT:
A hematological specimen for classifying and counting leukocytes with a flow cytometer is prepared. A sample to be assayed is prepared by eliminating influences of erythrocytes from a hematological sample without changing leukocytes morphologically by adding a first aqueous solution of a low osmotic pressure including a buffer for adjusting the pH value within an acidic region and a second aqueous solution including an osmolarity compensating agent and a buffer for giving pH value suitable for staining, optionally further adding a salt, which dissociates into ions in aqueous solutions so as to control the electrical conductivity of the aqueous solution at a preferable level, while damaging the cell membranes of erythroblasts contained in said sample; and staining the leukocytes with at least four dyes including Astrazon Yellow 3G and Neutral Red. Thus leukocytes contained in the hematological sample can be classified into at least eight groups including immature granulocytes, erythroblasts, basophils, eosinophils, lymphocytes, monocytes and neutrophils, or nine groups invoving one having blasts in addition to the above-mentioned eight groups, by assaying a single specimen with a flow cytometer.

REFERENCES:
patent: 3906120 (1975-09-01), Geating
patent: 4751179 (1988-06-01), Ledis et al.
patent: 4978624 (1990-12-01), Cremins et al.
patent: 5039613 (1991-08-01), Matsuda et al.
patent: 5122453 (1992-06-01), Martin et al.
David Freifelder, "Molecular Biology", 1987 p. 713.
Frankel et al., "Gradwohl's Clinical Laboratory Methods and Diagnosis" 1970, p. 508.

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