Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...
Patent
1999-05-27
2000-11-14
Brusca, John S.
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
435236, 435237, 435238, 435239, C12N 700, C12N 701, C12N 704, C12N 708, C12N 706
Patent
active
061468746
ABSTRACT:
Disclosed are methods for the isolation and purification of high-titer recombinant adeno-associated virus (rAAV) compositions. Also disclosed are methods for reducing or eliminating the concentration of helper adenovirus in rAAV samples. Methods are disclosed that provide highly-purified rAAV stocks having titers up to about 10.sup.13 particles/ml at particle-to-infectivity ratios of less than 100 in processes that are accomplished about 24 hours or less.
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International Search Report for Application No. PCT/US99/11945 dated Oct. 12, 1999.
Byrne Barry J.
Muzyczka Nicholas
Zolotukhin Sergei
Brusca John S.
Siu Stephen
University of Florida
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