Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1992-12-16
1995-12-26
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 691, 435 6, C12P 2100, C12N 900, C12Q 168
Patent
active
054787300
ABSTRACT:
Cell-free production of protein is provided by employing a barrier which defines a reaction region for maintaining the high molecular weight components of the cell-free system while providing means for replenishing loss of low molecular weight components and removing low molecular weight products. Various configurations may be employed for continuous removal and addition of material during the polypeptide synthesis. Conveniently, porous membranes, hollow fibers, or other barriers may be employed. High ratios of peptide to RNA template result.
REFERENCES:
patent: 4225671 (1980-09-01), Puchinger et al.
patent: 4668624 (1987-05-01), Roberts
Jagus, R.; Methods Enzymol 152:267-276 (1987).
Baranov, V. I. et al.; Gene 84:463-466 (1989).
Spirin, A. S.; Science 244:1162-1164 (1988).
Gisby, P. E. et al.; Methods Enzymol. 135:440-454 (1987).
Kigawa, T. et al.; J. Biochem. 110:166-168 (1991).
Ryabova, L. A. et al.; Nucleic Acids Res. 17:4412 (1989).
Lim et al., J. Pharm. Sci.. 70:351-354.
Bucke, Meth. Enzymol. 135:175-188.
Alakhov July B.
Baranov Vladimir I.
Morozov Igor J.
Ovodov Sergei J.
Ryabova Ljubov A.
Dehlinger Peter J.
Grimes Eric
Institute of Protein Research
Wax Robert A.
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