Method of preparing polynucleotide fragments for use in...

Details Method of preparing polynucleotide fragments for use in... Method of preparing polynucleotide fragments for use in...

2007-12-04

2007-12-04

Sullivan, Daniel M (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S441000, C435S442000, C435S443000, C435S444000, C435S445000

Reexamination Certificate

active

10713006

ABSTRACT:
The invention relates to a fragmentation process that depends on mismatches between two strands of parental polynucleotides. One embodiment, comprises a method for preparing polynucleotide fragments of DNA comprising formation of heteroduplex molecules by hybridizing polynucleotides. The invention also provides a method and process of forming fragments which can be used with any shuffling process or combination of shuffling processes.

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Stemmer et al. Proc. Natl. Acad. Sci. 1994; 91: 10747-51.
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Stemmer, W., “DNA shuffling by random fragmentation for molecular evolution”,National Academy of Sciences(Oct. 1, 1994), vol. 91, pp. 10747-10751, Washington. XP002087463.

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