Method of preparing plasmid having both expressing ability of re

Details Method of preparing plasmid having both expressing ability of re Method of preparing plasmid having both expressing ability of re

1996-07-19

2000-02-01

Brusca, John S.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4353201, 536 2372, C12N 1564, C12N 1563, C12N 1533

Patent

active

060201711

ABSTRACT:
The present invention provides a method of preparing a plasmid having both the ability of expressing retroviral genes and the ability of effecting the after-translational processing of the encoded products, and the resultant plasmid and the expression products thereof. The method of the present invention is to prepare a plasmid by insertion-linking a cDNA fragment containing at least the protease gene from among retroviral genes with a highly expressing gene or a gene direct expressing vector, thereby causing expression of the retroviral genes, and at the same time, to process said expression product itself with the protease in that expression product, thereby mass-producing three kinds of core protein encoded by vaq gene, and three kinds of enzymes encoded by pol gene, in the form of individual single mature or active proteins. Alternatively, the method of the present invention is to prepare a plasmid in which a cDNA fragment containing HIV nef gene region is inserted into a highly expressing gene and a gene direct expression vector, thereby mass-producing Nef protein which is the expression product of nef gene.

REFERENCES:
patent: 5093241 (1992-03-01), Bennett et al.
Guy et al. HIV F/3' orf encodes a phosphorylated GTP-binding resembling an ncogene product. Nature vol. 330 pp. 266-269, 1987.
Studier et al. Use of bacteriophage T7 polyberase to direct selective high-level expression of cloned genes J. Mol. Biol. vol. 189 113-130, 1986.
Wertman et al. Nucleotide sequence binding specificity of the lexA repressor of Escherichia coli J. Bact. vol. 163 376-384, 1985.
U. Ruther et al. et al. (1983) The Embo Journal 2(10): pp. 1791-1794.
S. Tabor et al. et al. (1985) Proc. Natl. Acad. Sci. USA 82: pp. 1074-1078.
G.C. Dubois et al. (1988) Aids Research and Human Retroviruses 4(6): pp. 419-431.
S.F.J. LeGrice et al. (1987) Gene 55: pp. 95-103.
A. Leuthardt et al. (1988) Gene 68: pp. 35-42.
C. DeBouck et al. (1987) Proc. Natl. Acad. Sci. USA 84: pp. 8903-8906.
S.R. Hammes et al. (1989) Proc. Natl. Acad. Sci. USA 86: 9549-9553.

Affiliated with

Also associated with

Rating

Method of preparing plasmid having both expressing ability of re does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of preparing plasmid having both expressing ability of re, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of preparing plasmid having both expressing ability of re will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-936611