Method of preparing plasmid having both expressing ability of re

Details Method of preparing plasmid having both expressing ability of re Method of preparing plasmid having both expressing ability of re

1994-02-25

1997-03-11

Elliott, George C.

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 435 712, 4351721, 435212, 4352351, 536 2372, C12P 2100, C12N 1500, C12N 950, C07H 2104

Patent

active

056100671

ABSTRACT:
The present invention provides a method of preparing a plasmid having both the ability of expressing retroviral genes and the ability of effecting the after-translational processing of the encoded products, and the resultant plasmid and the expression products thereof. The method of the present invention is to prepare a plasmid by insertion-linking a cDNA fragment containing at least the protease gene from among-retroviral genes with a highly expressing gene or a gene direct expressing vector, thereby causing expression of the retroviral genes, and at the same time, to process said expression product itself with the protease in that expression product, thereby mass-producing three kinds of core protein encoded by gag gene, and three kinds of enzymes encoded by pol gene, in the form of individual single mature or active proteins. Alternatively, the method of the present invention is to prepare a plasmid in which a cDNA fragment containing HIV nef gene region is inserted into a highly expressing gene and a gene direct expression vector, thereby mass-producing Nef protein which is the expression product of nef gene.

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