Method of preparing nutrient medium from litter-free liquid manu

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

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Details

435243, 435256, 435942, 426 62, 426807, A23K 100

Patent

active

042256763

ABSTRACT:
According to the proposed method, the starting material is separated into ncentrated suspension and clarified liquid fraction. The concentrated suspension is heated and hydrolyzed under excess pressure in the presence of a mineral acid as a catalyst. The clarified liquid fraction of the starting material is sterilized and mixed with the hydrolysate. The mixture is then neutralized to the required pH. The neutralized mixture is then separated from harmful volatile admixtures and cooled to obtain nutrient medium suitable for cultivating microorganisms. To separate the starting material into concentrated suspension and clarified liquid fraction, to hydrolyze the concentrated suspension, to sterilize the clarified liquid fraction of the starting material, to mix the hydrolysate with the sterilized clarified liquid and to neutralize the mixture, to remove harmful volatile substances from the neutralized mixture and to cool the final neutralized mixture to prepare nutrient medium for cultivating microorganisms, the plant is provided with the appropriate devices and vessels. In order to mix the sterilized liquid fraction of the starting material with the hydrolysate and subsequently to neutralize the mixture, the sterilizer is connected with the device for separation of the starting material and the neutralization tank; and in order to hydrolyze the concentrated suspension use is made of a hydrolyzer having a closed cylinder, lined on the inside with a heat- and acid-resistant material. Connections are mounted on the hydrolyzer for delivery of concentrated suspension and a mineral acid, for delivery of heating steam into the lower part of the apparatus, for discharging the hydrolyzate from the hydrolyzer, and for releasing excess steam from the hydrolyzer.

REFERENCES:
patent: 3375116 (1968-03-01), Anthony
patent: 3860487 (1975-01-01), Emanuel
patent: 3865568 (1975-02-01), Kratzer
patent: 3875319 (1975-04-01), Seckler et al.
patent: 4018899 (1977-04-01), Seckler et al.
patent: 4119495 (1978-10-01), Belyaev et al.

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