Method of preparing nucleotide probes using a bridging complemen

Details Method of preparing nucleotide probes using a bridging complemen Method of preparing nucleotide probes using a bridging complemen

1994-09-08

1996-07-23

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

536 237, 536 2372, 536 2432, C12P 1934, C07H 2104

Patent

active

055388728

ABSTRACT:
The present invention provides labeled nucleotide probes and a procedure for synthesis of the labeled nucleotide probe by a coupling or joinder of two components of the probe, namely a target recognition sequence moiety and a signal generating moiety, through the use of a bridging complement that holds the two moieties in such a position as to allow formation of a sugar-phosphate chemical linkage between the 3' end of one moiety and the 5' end of the other moiety.

REFERENCES:
patent: 4563419 (1986-01-01), Rarki et al.
patent: 4656127 (1987-04-01), Mundy
patent: 4699876 (1987-10-01), Libeskind
patent: 4711955 (1918-12-01), Ward et al.
patent: 4794082 (1988-12-01), Sigler
patent: 4797355 (1989-01-01), Stabinsky
USB, Molecular Biology Reagents/Protocols 1992, pp. 114-115.
Matthews and Kricka, "Review: Analytical Strategies for the Use of DNA Probes", Analytical Biochemistry 169:1-25 (Feb. 1988).
Sambrook, J. et al., "Molecular Cloning", A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press 1989.
Sanchez-Pescador, R. et al., "Rapid Chemiluminescent Nucleic Acid Assays for Detection of TEM-1-.beta.-Lactamase-Mediated Penicillin Resistance in Neisseria gonorrhoeae and Other Bacteria", J. of Clinical Microbiology 26:1934-1938 (1988).

Affiliated with

Also associated with

Rating

Method of preparing nucleotide probes using a bridging complemen does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of preparing nucleotide probes using a bridging complemen, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of preparing nucleotide probes using a bridging complemen will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-712261