Method of preparing nucleic acids having an undefined nucleotide

Details Method of preparing nucleic acids having an undefined nucleotide Method of preparing nucleic acids having an undefined nucleotide

1994-10-17

1997-02-04

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C12P 1934, C12Q 168

Patent

active

055996965

ABSTRACT:
A method for amplifying specific nucleic acid fragments having an undefined nucleotide sequence wherein said nucleic acid fragments are present in a mixture of nucleic acids. Blunt ended linkers with known nucleotide sequences are used in the method to isolate and distinguish the specific undefined nucleic acid fragments from the rest of the nucleic acid. Primers which hybridize with the known linker are then used as part of a polymerase chain reaction procedure to amplify the specific nucleic acid fragment which includes the undefined nucleotide sequence.

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Frohman et al., Rapid Production of Full-length cDNAs from Rare Transcripts; Amplification Using a Single Gene-specific Oligonucleotide Primer, Proc. Natl. Acad. Sci. USA 85:8998-9002 (1988).
Loh et al., Polymerase Chain Reaction with Single-Sided Specificity: Analysis of T Cell Receptor & Chain, Science 243:217-220 (1989).
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Maniatis et al, Molecular Cloning--A Laboratory Manual, Cold Spring Harbor Laboratory, 1982, pp. 133, 146, 219, 220.
New England BioLabs Price List, Aug. 1989.

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