Method of preparing nucleic acids for detection

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S288200, C435S288300, C435S288400, C435S305100, C435S305200, C435S305300, C435S287200, C422S091000, C422S105000

Reexamination Certificate

active

10703368

ABSTRACT:
A method and apparatus are provided for preparing a test sample for detecting a predetermined target nucleic acid. The method includes the steps of providing a test probe comprising an oligonucleotide attached to a nanoparticle and providing a hybridization unit containing the test sample and the test probe, wherein said hybridization unit further includes a target sample substrate and a distribution manifold coupled to a first side of the substrate. The method further includes the steps of clamping a processing fluids manifold to the distribution manifold of the hybridization unit, denaturing the test sample and preparing the test sample for detecting the predetermined target nucleic acid by pumping a plurality of processing fluids between the processing fluids source manifold and distribution manifold to hybridize the test probe and predetermined target nucleic acid to the target sample substrate, to wash the hybridized sample and to amplify a detectable parameter of the hybridized sample.

REFERENCES:
patent: 5587128 (1996-12-01), Wilding et al.
patent: 6340598 (2002-01-01), Herron et al.
patent: 6403379 (2002-06-01), Munson et al.
patent: 6444461 (2002-09-01), Knapp et al.
patent: 2001/0046702 (2001-11-01), Schembri
patent: 2004/0053268 (2004-03-01), Karlsen
patent: 2004/0087033 (2004-05-01), Schembri
patent: 2004/0229247 (2004-11-01), DeBoer et al.
patent: 2004/0248318 (2004-12-01), Weinberger et al.
patent: 2005/0214854 (2005-09-01), Dahm et al.
patent: 2005/0271551 (2005-12-01), Shumate et al.
patent: 2006/0051798 (2006-03-01), Mirkin et al.
patent: 2007/0098600 (2007-05-01), Kayyem

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method of preparing nucleic acids for detection does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of preparing nucleic acids for detection, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of preparing nucleic acids for detection will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3940918

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.