Method of preparing murine monoclonal antibodies against cell-fr

Details Method of preparing murine monoclonal antibodies against cell-fr Method of preparing murine monoclonal antibodies against cell-fr

1983-11-02

1986-06-24

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435240, 435241, 435948, 530387, 530806, 530351, C12P 2100, C12N 500, C12N 502, C12R 191

Patent

active

045967741

ABSTRACT:
A method of preparing simultaneously monoclonal antibodies specific for different cell-free products of activated human T lymphocytes is disclosed. Human T cells are activated in a medium supplemented with mouse serum rather than conventional calf serum. A supernatant prepared from the activated T cells is used to immunize mice. The dominant immunogens in the supernatant are the cell-free products of human T lymphocytes. The yield of hybrid cells which produce products reactive with cell-free products of human T lymphocytes is enhanced by injecting the immunized mice with a supernatant from mitogen-activated murine splenocytes. In addition, a novel radioimmunoadsorbent assay for screening hybrids to detect production of monoclonal antibodies reactive with cell-free products of human T lymphocytes is disclosed.

REFERENCES:
patent: 4401756 (1983-08-01), Gillis
patent: 4411992 (1983-10-01), Gillis
patent: 4411993 (1983-10-01), Gillis
patent: 4468460 (1984-08-01), Kumamoto
Neta et al, from Lymphokines vol. 2, Edgar Pick, Editor, pp. 296-301, 1981.

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