Method of preparing membrane filters for transmission electron m

Details Method of preparing membrane filters for transmission electron m Method of preparing membrane filters for transmission electron m

1990-06-12

1991-04-02

Berman, Jack I.

Radiant energy

Methods including separation or nonradiant treatment of test...

250307, 436176, 436178, G01N 128

Patent

active

050049208

ABSTRACT:
This application relates to a method for preparing specimens for viewing particles and/or fibers captured upon membrane filters with a TEM. The method comprises mounting a section of the membrane filter on a glass slide with a quick drying liquid applied between the filter membrane and the slide which liquid will not release the filter membrane when the slide is immersed in a selected solvent and such that the liquid will not render the filter membrane resistant to the selected solvent. Next, the filter section surface is coated with carbon to replicate the surface of the membrane filter and the particles and fibers thereupon. The coated membrane is then immersed in the selected solvent which will attack the filter material causing the carbon film squares to float off of the memberane filter.

REFERENCES:
patent: 2875341 (1959-02-01), Nesh
Pattnaik et al, Scanning Electron Microscopy 11976 (Part III), Proceedings of the Workshop on Techniques for Particulate Matter Studies in SEM, Apr. 1976, pp. 441-450.
The Journal of General Microbiology, vol. 17, No. 1, Aug. 1957, pp. 75-79, "An Electron Microscope Study of the Spores of Some Species of the Genus Bacillus using Carbon Replicas", D. E. Bradley and D. J. Williams.
Industrial Lab. (U.S.A.), vol. 45, No. 3 (1979), "Rapid Aimed Preparation of Replicas (Exchange of Experience)", A. Kornienko and A. A. Nikolaenko.

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