Method of preparing ligands for hydrophobic interaction...

Details Method of preparing ligands for hydrophobic interaction... Method of preparing ligands for hydrophobic interaction...

2008-01-22

2008-01-22

Therkorn, Ernest G. (Department: 1797)

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

C210S656000, C210S198200, C210S502100, C502S401000, C502S439000

Reexamination Certificate

active

07320755

ABSTRACT:
The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.

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Feist, P., et al., “Sulfhydrylcellulose: A New Medium for Chromatography of Mercurated Polynucleotides”,Biochemistry, vol. 20, No. 15, 1981, p. 4243-4246.

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