Method of preparing hybridomas and of purifying immunogenic mate

Details Method of preparing hybridomas and of purifying immunogenic mate Method of preparing hybridomas and of purifying immunogenic mate

1982-03-01

1984-12-11

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435240, 435241, 435 68, 436548, 935 90, 935 95, 935104, C12N 1500, C12N 500, C12N 502, C12P 2100, G01N 3554

Patent

active

044878338

ABSTRACT:
In a method for preparing a monoclonal hybridoma which secretes antibodies against an immunogenic material, comprising sensitizing an appropriate source with an immunizing amount of a preparation of the immunogenic material, obtaining from the source lymphocytes having immune activity against the immunogenic material, and fusing the lymphocytes with an appropriate cell line to thereby form mixed hybridomas, the improvement wherein: the immunogenic material is present in the immunizing preparation in an amount not larger than 5% by weight, and wherein, after formation of the mixed hybridomas but prior to cloning, the method comprises the steps of: raising ascites fluid with the mixed hybridomas, then detecting immune specificity against the immunogenic material in the ascites raised fluid; and thereafter cloning the ascites raised cells which show immune specificity against the material.

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