Method of preparing highly purified, stroma-free, non-hepatitic

Details Method of preparing highly purified, stroma-free, non-hepatitic Method of preparing highly purified, stroma-free, non-hepatitic

1984-05-29

1985-07-02

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

2601125R, 424101, 514 2, A61K 3518, A61K 3702, C07G 700, C07C10352

Patent

active

045267154

ABSTRACT:
To provide a method that allows the preparation, not only from human blood but also from animal blood and in quantities large enough for clinical applications, of highly purified hemoglobin solutions that can be employed for the treatment of humans and that are free of plasma proteins and residual stroma lipids (a) a starting material that contains erythrocytes is pumped over a filter unit that has a pore size between 0.1 and 1.2 .mu.m in circulation against an isotonic washing solution, (b) the product of (a) is hemolyzed and pumped over an ultrafiltration unit that has a permeability of 80,000 to 100,000 D in circulation against water, and (c) the ultrafiltrate obtained in stage (b) is pumped and diafiltered over an ultrafiltration unit that has a permeability of 10,000 to 50,000 D in circulation against water, whereby concentration is conducted before and/or after diafiltration.

REFERENCES:
patent: 3864478 (1975-02-01), Bonhard
patent: 4001200 (1977-01-01), Bonsen et al.
patent: 4136093 (1979-01-01), Bonhard et al.
patent: 4376059 (1983-03-01), Davis et al.
patent: 4401652 (1983-08-01), Simmonds et al.
patent: 4439357 (1984-03-01), Bonhard et al.
F. DeVenuto et al., Characteristics of Stroma-Free Hemoglobin Prepared by Crystallization, Letterman Army Institute of Research, CA, 1976, pp. 509-516.
F. DeVenuto et al., Appraisal of Hemoglobin Solution as a Blood Substitute, Letterman Army Institute of Research, CA, (Surgery, Gynecology & Obstetrics)-Sep. 1979, vol. 149, pp. 417-435.
Mario Feola et al., Development of a Bovine Stroma-Free Hemoglobin Solution as a Blood Substitute, Gynecology & Obstetrics, 11/83, vol. 157, No. 5, pp. 399-408.

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