Method of preparing botulinum neurotoxin type E light chain

Details Method of preparing botulinum neurotoxin type E light chain Method of preparing botulinum neurotoxin type E light chain

2008-11-18

2010-11-16

Mondesi, Robert B (Department: 1645)

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

C435S071300

Reexamination Certificate

active

07833524

ABSTRACT:
The present invention provides a preparation of botulinum toxin light chain type A or E, wherein the preparation is both catalytically active and soluble. Preferably, the preparation consists essentially of amino acid residues 1 through 425 of the botulinum toxin light chain type A. A method of screening inhibitors is also provided, wherein the method comprises exposing a test inhibitor to the preparation of botulinum toxin light chain type A and evaluating the biological activity of the preparation. In another embodiment, a method of providing a catalytically active, soluble preparation of botulinum toxin light chain, type A is provided, wherein the method comprises obtaining an expression vector comprising a DNA sequence encoding amino acid residues 1-425 and expressing a polypeptide.

REFERENCES:
Wang et al. (Appl. Environ. Microbiol., 66:4992-4997).
Fujii et al. (Microbiol. Immunol., 36:213-220, 1992).
Agarwal et al. (Biochem., 43:6637-6644, 2004).
Baldwin et al., Prot. Exp. Purif., 37:187-195, 2004.
CRC Handbook of Chemistry and Physics, 78th edition, CRC Press, 1997, p. 2-53.
Cai S & Singh B, Biochemistry 40:4693-4702 (2001).
Dineen S, et al., Curr. Microbiol. 46:345-352 (2003).
Kadkhodayan S, et al., Protein Expr. Purif. 19: 125-130 (2000).
Kurazono H, et al., J. Biol. Chem. 267: 1472 1-14729 (1992).
LaPenotiere H, et al., Toxicon 33: 1383-1386 (1999).
Li L & Singh B, Protein Expr. Purif. 17:339-344 (1999).
Rossetto 0, et al., Toxicon 39: 1 15 1-1 159 (2001).
Zhou L, et al.,Biochemistry 34: 15 175- 15 18 1 (1995).
Li Y, et al., Biochemistry 33:7014-7020 (1994).

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