Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1996-09-17
1999-01-26
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435325, 4351723, 536 232, 536 235, C12N 902
Patent
active
058637761
ABSTRACT:
The present invention provides a method of preparing apoenzyme of a riboflavin-dependent enzyme, comprising the steps of: growing COS-7 cells in a riboflavin-free medium; introducing a cDNA encoding a riboflavin-dependent enzyme; and expressing said cDNA. Also provided is a method of screening for an inhibitor of a flavin dependent enzyme, comprising the steps of;
contracting a potential inhibitor of a flavin dependent enzyme with the cell line of claim 7 in the presence of riboflavin, FMN or Fad; and measuring the enzymatic activity of the flavin dependent enzyme.
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Bach et al. "cDNA cloning of human liver monoamine oxidase A and B: molecular basis of differences in enzymatic properties" Proc. Natl. Acad. Sci. USA 85, 4934-4938, Jul. 1988.
Potter et al. "Enhancer-dependent expression of human k immunoglobulin genes . . . by electroporation" Proc. Natl. Acad. Sci. USA 81, 7161-7165, Nov. 1984.
Abell Creed W.
Kwan Sau-Wah
Lewis Duane A.
Zhou Binhua
Adler Benjamin Aaron
Nashed Nashaat T.
Research Development Foundation
Wax Robert A.
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