Method of preparing and activating samples for radioimmunoassay

Details Method of preparing and activating samples for radioimmunoassay Method of preparing and activating samples for radioimmunoassay

1995-12-29

1997-09-16

Pyon, Harold

Chemistry: analytical and immunological testing

Including sample preparation

Dilution

436177, 436180, 422 681, 422 72, 422 71, G01N 110

Patent

active

056680163

ABSTRACT:
A method of activating target proteins in a biologic sample for radioimmunoassay or other immunoassay by adding the sample to a diluent containing no calcium or magnesium, heating the mixture in a boiling water bath for a designated period of time, cooling the sample, centrifuging the mixture to isolate the activated protein containing supernatant, and then vortexing the supernatant to ensure homogeneity of the protein.

REFERENCES:
patent: 4999304 (1991-03-01), Robertson
patent: 5243041 (1993-09-01), Fernandez-Pol
patent: 5367063 (1994-11-01), Bomalaski et al.
patent: 5395753 (1995-03-01), Prakash
patent: 5494803 (1996-02-01), Carbonell et al.
patent: 5530100 (1996-06-01), Darling et al.
Jose A. Fernandez-Pol et al.,; Journal of Tumor Marker Oncology; Jun. 1995; Abstract 49.

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