Method of preparing an active human neutrophil chemotactic facto

Details Method of preparing an active human neutrophil chemotactic facto Method of preparing an active human neutrophil chemotactic facto

1992-03-23

1995-03-28

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351223, 43525233, 4353201, 530412, 530351, C12P 2102, C12P 1934, C12N 1500, C12N 700

Patent

active

054016436

ABSTRACT:
A simple and efficient method of preparing active recombinant human neutrophil chemotactic factor. The human neutrophil chemotactic factor (NCF) is solubilized from a transformant cell homogenate using urea or guanidine hydrochloride. The polypeptide is further purified from the solution using conventional protocols such as CM-Sepharose CL-6B, HW-55 column, etc.. By using this method, large amounts of active human NCF can be recovered and purified.

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