Method of preparing alpha-1-proteinase inhibitor and antithrombi

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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424101, 514 8, 514 21, 530380, 530392, 530395, 530830, C07K 328, C07K 320, C07K 1506

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046562540

ABSTRACT:
There is disclosed an improved method for separating one of alpha-1-proteinase inhibitor (also known as alpha-1 antitrypsin) and antithrombin-III from an aqueous solution of plasma proteins containing the same, such as from Cohn Fraction IV-1, Cohn Fraction IV, reworks of Cohn Fraction IV and IV-1, Cohn Effluent II & III and Cohn Effluent I. The method includes the steps of first holding an aqueous solution of plasma proteins containing one of alpha-1-proteinase inhibitor and antithrombin-III in a relatively large volume of buffer solution as solvent and at a pH adjusted to be relatively basic when compared to conditions heretofore known, and at a temperature in the range of from 2.degree.-50.degree. C. for a period of about 0.2-24 hours. Following the above treatment, alpha-1-proteinase inhibitor and antithrombin-III are obtained by applying conventional techniques to the resulting solution. Accordingly, the solution is then mixed with a polyalkylene glycol, e.g. polyethylene glycol, in the range of from as low as 8% to as high as 23% (w/v) based on volume of solution to selectively precipitate unwanted proteins. The alpha-1-proteinase inhibitor is then separated from the supernatant solution by adsorption on an anion exchange resin or by precipitation by further addition of a polyalkylene glycol, e.g. polyethylene glycol. The method of the invention provides an improvement in the yield of alpha-1-proteinase inhibitor.

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Preparative Biochemistry 5(4), 333-348 (1975), Glaser et al.
Analytical Biochemistry, 124, 364-371 (1982), Glaser et al.

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