Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1996-08-22
1999-11-02
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 691, 4352523, 4353201, 435471, 435832, 435849, 435874, 435886, 435913, 435911, 536 232, C12N 920, C12N 120, C12N 100, C07H 2104
Patent
active
059768550
ABSTRACT:
The present invention relates to a method of preparing a variant of a parent lipolytic enzyme, comprising (a) subjecting a DNA sequence encoding the parent lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which has a decreased dependance to calcium and/or an improved tolerance towards a detergent or a detergent component as compared to the parent lipolytic enzyme.
REFERENCES:
patent: 4600693 (1986-07-01), Kindle et al.
Abstract--Dialog; File 155, Medline, Dialog Accession No. 06804243.
Clausen Ib Groth
Okkels Jens Sigurd
Svendsen Allan
Thellersen Marianne
Lambiris, Esq. Elias J.
Novo Nordisk A S
Saidha Tekchand
Wax Robert A.
Zelson Esq. Steve T.
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