Method of preparing a test strip for alcohol testing

Details Method of preparing a test strip for alcohol testing Method of preparing a test strip for alcohol testing

1985-02-20

1988-11-22

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 26, 435 25, 435192, 435805, 436132, 436169, 436900, 436904, 422 56, 422 57, C12Q 128

Patent

active

047865962

ABSTRACT:
Ethyl alcohol test strip fabricating and use techniques for testing an individual's saliva to determine his sobriety are disclosed. The test strips employ an alcohol oxidase, Peroxidase and an hydrogen donor indicator such as Tetraalkylbenzidine in a carrier matrix supported on the strip with the alcohol oxidase functioning as a catalyst to convert any ethanol present along with ambient oxygen to acetaldehyde and hydrogen peroxide. The peroxidase function as catalyst to induce a color change in the hydrogen donor and convert the hydrogen peroxide to water. The method of fabricating the test strip involves multiple steps of reagent application and hot air drying.

REFERENCES:
patent: 4110079 (1978-08-01), Schaeffer et al.
patent: 4361648 (1982-11-01), Shuenn-Tzong
Singh et al., Anolytica Chimica Acta (1980), vol. 115, pp. 401-405.
Janssen et al., Biochimica et Biophysica Acta (1968), vol. 151, pp. 330-342.

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