Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Patent
1997-12-05
2000-02-01
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
4351722, 436518, 436548, 5303881, 5303882, C12P 2104, C12N 1500, G01N 33543, G01N 3353
Patent
active
060201703
ABSTRACT:
The present invention relates to an efficient method of producing monoclonal antibodies against surface antigens of cells and viruses. The method accommodates for antigens which are present in only relatively small amounts, or antigens of which only very small amounts are available or antigens which easily lose their in vivo conformation. Thus the method according to the invention comprises a series of steps, comprising a step in which B-cells from a mammal injected with surface antigen-comprising material are enriched with respect to the relative number of specific B-cells and a step which comprises a small-scale fusion technique.
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Akzo Nobel N.V.
Blackstone William M.
Ceperley Mary E.
Devi S.
Sullivan Michael G.
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